Gelatin-based Hydrogel Degradation and Tissue Interaction in vivo: Insights from Multimodal Preclinical Imaging in Immunocompetent Nude Mice
نویسندگان
چکیده
Hydrogels based on gelatin have evolved as promising multifunctional biomaterials. Gelatin is crosslinked with lysine diisocyanate ethyl ester (LDI) and the molar ratio of gelatin and LDI in the starting material mixture determines elastic properties of the resulting hydrogel. In order to investigate the clinical potential of these biopolymers, hydrogels with different ratios of gelatin and diisocyanate (3-fold (G10_LNCO3) and 8-fold (G10_LNCO8) molar excess of isocyanate groups) were subcutaneously implanted in mice (uni- or bilateral implantation). Degradation and biomaterial-tissue-interaction were investigated in vivo (MRI, optical imaging, PET) and ex vivo (autoradiography, histology, serum analysis). Multimodal imaging revealed that the number of covalent net points correlates well with degradation time, which allows for targeted modification of hydrogels based on properties of the tissue to be replaced. Importantly, the degradation time was also dependent on the number of implants per animal. Despite local mechanisms of tissue remodeling no adverse tissue responses could be observed neither locally nor systemically. Finally, this preclinical investigation in immunocompetent mice clearly demonstrated a complete restoration of the original healthy tissue.
منابع مشابه
Co-Transplantation of VEGF-Expressing Human Embryonic Stem Cell Derived Mesenchymal Stem Cells to Enhance Islet Revascularization in Diabetic Nude Mice
Background: Pancreatic islet transplantation has emerged as a promising treatment for type I diabetes. However, its efficacy is severely hampered due to poor islet engraftment and revascularization, which have been resulted to partially loss of transplanted islets. It has been shown that local delivery of vascular endothelial growth factor (VEGF) could accelerate transplanted islet revasculari...
متن کاملSynthesis and Swelling Behavior of Gelatin-Based Hydrogel Nanocomposites
In this work, a series of hydrogel nanocomposites were prepared by grafting acrylic acid and acrylamide on gelatin in the presence of Na-montmorillonite (Na-MMT) nanoparticles. The characterizations of hydrogel nanocomposites were examined by swelling experiments, Fourier transform infrared (FT-IR) spectroscopy, X-Ray diffraction (XRD) patterns and thermogravimetric analysis (TGA). Scanning ele...
متن کاملPreparation of polymer-based multimodal imaging agent to visualize the process of bone regeneration.
The objective of this study is to design a new multimodal imaging system for the evaluation of bone regeneration process. Pamidronate (PA) of bisphosphonates with a high affinity for hydroxyapatite, was introduced to pullulan with different molecular weights (PA-pullulan). Then, two probes for fluorescence and magnetic resonance (MR) imagings were introduced into the PA-pullulan to prepare the ...
متن کاملSynthesis and Application of New Gadolinium-Porphyrins as Potential MR Imaging Contrast Agents for Cancer Detection in Nude Mice
Two new potential magnetic resonance imaging contrast agents, Gd-hematoporphyrin (Gd-H) and Gd-tetra-carboranylmethoxyphenyl-porphyrin (Gd TCP), were synthesized and applied to nude mice with human melanoma (MM 138) xenografts. These agents showed a high relaxivity because of their greater potential to coordinate water molecules. The reduction of T1 relaxation times of 16 and 21% was observed i...
متن کاملA cisplatin slow-release hydrogel drug delivery system based on a formulation of the macrocycle cucurbit[7]uril, gelatin and polyvinyl alcohol.
The anticancer drug cisplatin was encapsulated within the cucurbit[7]uril macrocycle to form the host-guest complex: cisplatin@CB[7]. This was then incorporated into gelatin and 0-4% w/v polyvinyl alcohol (PVA)-based hydrogels as slow release drug delivery vehicles. The hydrogels demonstrated predicable swelling and disintegration dependent on the PVA concentration. The hydrogel with the highes...
متن کامل